Skip to main content
. 2010 Dec 28;2(2):229–237. doi: 10.3892/etm.2010.189

Figure 1.

Figure 1.

Figure 1.

Medulloblastoma cell lines express cell surface HSPGs and GEF-H1 is a component of a SDC CT-binding complex. (A) Semi-quantitative RT-PCR analysis of D283 and D721 cells. GAPDH was used as a positive control. Mastermix with the primer pair was used as a negative control and a test for contamination. PCR product sizes: GAPDH, 611 bp; SDC1, 725 bp; SDC2, 548 bp; SDC3, 824 bp; SDC4 500 bp; GPC1, 849 bp; GPC2, 602 bp; GPC3, 488 bp; GPC4, 517 bp; GPC5, 569 bp; GPC6, 573 bp. (B) SDS-PAGE of GST pulldown eluates. The arrow indicates the endogenously-expressed SDC1/4 CT binding protein that was excised from a Coomassie blue-stained gel of GST pulldown eluates. (C) GEF-H1 is identified by MALDI-TOF MS/MS as a SDC4 CT-associated protein from the D283 cell lysate, per B. (D) Representative Western blotting detecting endogenous SDC1 (core protein), SDC4 (core protein) and GEF-H1 in poorly (D283) and highly (D721) invasive MB cells. β-actin was used as a control for equal loading.