Figure 3.

Heparanase (HPSE) pre-treatment modulates Shh/Wnt3a-dependent expression of Gli genes in human MB cells. Cells (D283 and D721) were pre-treated without (−) or with (+) active rhHPSE (200 ng/ml), and subsequently treated with Shh and/or Wnt3a (100 ng/ml each), RNA was isolated, and semi-quantitative RT-PCR was performed (Ctrl, not growth factor-treated). GAPDH was used as a positive control. Mastermix without the primer pair was used as a negative control and a test for contamination. PCR product sizes: GAPDH, 611 bp; Gli1, 572 bp; Gli2, 492 bp; Gli3, 416 bp.