Skip to main content
. 2010 Sep 29;31(3):819–831. doi: 10.1038/jcbfm.2010.163

Figure 4.

Figure 4

Efficiency of AQP4 inhibition with small interference RNA against AQP4 (siAQP4). (A) AQP4 expression in siGLO- and siAQP4-treated rats was analyzed by Western blot where a specific band at 30 kDa was observed (red) in siGLO-treated rats. The intensity of the signal was decreased in siAQP4-treated rats, with no change in the intensity of the actin band (green). (B) Expression of AQP4 (n=4) was decreased (0.9±0.09 arbitrary units, A.U.) in siAQP4 compared with siGLO-treated animals (1.22±0.11 A.U., *P<0.05, unpaired t-test). (C) AQP4 immunolabeling was also performed to examine variations in AQP4 expression in situ. (C1, C2) Confocal images of AQP4 staining in siGLO- (C1) and siAQP4 (C2)-treated rats showed a significant decrease in the intensity of AQP4 staining in the ipsilateral cortex of siAQP4-treated rats (P<0.05, unpaired t-test). (D) AQP4 labeling in the contralateral striatum of the siGLO- (D1) and siAQP4-treated rats (D2) revealed decreased AQP4 expression. These immunohistochemical results are in accordance with the Western blot decreases in AQP4 expression (Figures 2A and 2B). (E) AQP4 immunolabeling (n=6) was quantified using optical densitometry demonstrating a decrease in all brain areas (*P<0.05 and **P<0.001, unpaired t-test). The color reproduction of this figure is available on the html full text version of the manuscript.