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a and b Original recordings showing representative [Ca2+]i transients in response to stimulation of isolated detrusor cells with 100 μM bethanechol (BE) or ATP. c shows the peak values (average ± SEM) in response to BE, ATP and KCl (60 mM) in adult and aged cells. n = 14–33 cells from five to six animals (**P < 0.01, ***P < 0.001 aged vs adult)
