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. 2010 Aug;1(8):868–876. doi: 10.1177/1947601910383416

Figure 2.

Figure 2.

Andrographolide inhibits IL-6 expression. (A) Chemical structure of andrographolide. (B) Effect of andrographolide on IL-6 protein expression. DU145 and PC3 cells were treated with different doses of andrographolide for 24 hours, and the supernatants were used for detection of IL-6 protein expression by ELISA. The results were expressed as percentages compared to the vehicle control. (C) Time course effect of andrographolide on IL-6 protein expression. DU145 cells were cultured in 12-well plates. The media were changed when andrographolide (10 µM) was added to the cells. The media were collected at the time points indicated and used for IL-6 determination by ELISA. (D) Effect of andrographolide on IL-6 mRNA expression by qRT-PCR analysis. DU145 cells were treated with different concentrations of andrographolide as indicated. Total RNA was isolated and subjected to qRT-PCR analysis. Asterisks (*) indicate statistical significance.