Figure 4.

Andrographolide inhibits the growth of prostate cancer cells. (A) Andrographolide selectively inhibits the growth of DU145 and PC-3 prostate cancer cells over normal immortalized prostate epithelial cells. PC-3, DU145, or PZ-HPV7 cells were plated in 12-well plates and treated with various concentrations of andrographolide. After 48 hours, the cells were counted under a microscope. Results are expressed as the average percentage of live cells with standard deviation. Each bar is representative of 3 different experiments. Asterisks (*) indicate statistical significance. (B) Effect on apoptosis. Mononucleosomes and oligonucleosomes in the cytoplasmic fraction were analyzed by cell death detection ELISA as described in Materials and Methods. (C) Andrographolide inhibits DU145 penetration through Matrigel in invasion assay.