TABLE 4.
Summary of GISH assay for individual plants of the five selected BC3S1 families
| BC3S1 plant | Expected B chromosome | No. of red signals | Total chromosome count |
|---|---|---|---|
| IF−29–2 | B3/B7/B8 | 1 | 38 |
| IF−29–3 | B3/B7/B8 | 2 | 40 |
| IF−29–5 | B3/B7/B8 | 1 | 39 |
| IF−30–2 | B6 | 1 | 39 |
| IF−30–3 | B6 | 0 | 40 |
| IF−30–5 | B6 | 0 | 40 |
| IF−42–1 | B3/tip of B7 | 3 | 42 |
| IF−42–2 | B3/tip of B7 | Fragments | 36 |
| IF−103–1 | B7 | 1 | 37 |
| IF−103–2 | B7 | 1 | 37 |
| IF−115–1 | B3/B7 | 2 | 40 |
| IF−115–2 | B7 | 1 | 37 |
| IF−115–3 | B7 | 0 | 36 |
| IF−174–1 | B8 | 0 | 37 |
| IF−174–3 | B8 | 0 | 36 |
| IF−174–5 | B8 | 0 | 38 |
| Westar | None | 0 | 38 |
| Carinata | All | 8 | 34 |
Expected B-genome chromosome on the basis of SSR marker analysis, total chromosome number, and observed number of B. nigra genomic signals are presented.