Figure 4.—

Alignment of Chlamydomonas, Volvox, and Dunaliella CCS4 proteins. Sequences of C. reinhardtii (Crein, accession no. ADL27744), V. carteri (Vcart, accession no. FD920844.1), D. salina (Dsali1, accession no. BM447122.1; Dsali2, accession no. BM448413.1) CCS4 were aligned using the CLUSTALW algorithm (Blosum62 scoring matrix) in Bioedit. The alignment was edited using the GeneDoc multiple alignment editor. Strictly conserved or similar amino acids are on a solid background. The putative membrane anchor is boxed. The solid downward arrowhead indicates the position of the methionine in the truncated CCS4 form and the shaded downward arrowhead indicates the Q residue that is mutated to a stop codon in the ccs4-F2D8 strain.