FIG. 1.

The methods to characterize fibrin-based implants and neovascularization are depicted. (A) A compilation of images stitched together reveals the gross morphology of a typical fibrin implant (left). Candidate vessel structures identified by the presence of well-defined lumens (arrowheads, right) were counted to yield a quantitative metric of vessel formation. Higher magnification images show (B) typical implant orientation relative to the subcutaneous mouse tissue (F identifies fibrin) and (C) examples of lumen-containing structures that were quantified. Color images available online at www.liebertonline.com/tea.