Table 1.
Melting Temperatures of DNA/Polyamide Complexes for A • T and T • A Base Pairs at the Turn Position of Hairpin Polyamidesa
| Polyamides | A • T | T • A | ||
|---|---|---|---|---|
| 5′-CGA
TGTTCA
AGC-3′ |
5′-CGA
TGTTCT
AGC-3′ |
|||
| Tm/°C | ΔTm/°C | Tm/°C | ΔTm/°C | |
| — | 54.0 (±0.2) | — | n.d. | |
(1)
|
69.9 (±0.3) | 15.9 | n.d. | |
(2)
|
73.5 (±0.2) | 19.5 | n.d. | |
(3)
|
74.9 (±0.2) | 20.9 | n.d. | |
(4)
|
76.2 (±0.2) | 22.2 | n.d. | |
|
| ||||
| 5′-CGA
TGGTCA
AGC-3′ |
5′-CGA
TGGTCT
AGC-3′ |
|||
| — | 57.2 (±0.1) | — | 55.8 (±0.1) | — |
(5)
|
70.6 (±0.2) | 13.4 | 69.0 (±0.3) | 13.2 |
(6)
|
74.1 (±0.3) | 16.9 | 72.9 (±0.2) | 17.1 |
(7)
|
76.1 (±0.2) | 18.9 | 73.2 (±0.1) | 17.4 |
(8)
|
77.5 (±0.3) | 20.3 | 74.2 (±0.1) | 18.4 |
|
| ||||
| 5′-CGA
TGGGCA
AGC-3′ |
5′-CGA
TGGGCT
AGC-3′ |
|||
| — | 60.2 (±0.2) | — | 59.8 (±0.3) | — |
(9)
|
68.8 (±0.2) | 8.6 | 67.4 (±0.3) | 7.6 |
(10)
|
73.4 (±0.2) | 13.2 | 72.0 (±0.1) | 12.2 |
(11)
|
73.5 (±0.1) | 13.3 | 70.5 (±0.3) | 10.7 |
(12)
|
73.8 (±0.1) | 13.6 | 71.3 (±0.3) | 11.5 |
|
| ||||
| 5′-CGA
TGGGGA
AGC-3′ |
5′-CGA
TGGGGT
AGC-3′ |
|||
| — | 57.5 (±0.1) | — | 57.9 (±0.1) | — |
(13)
|
60.9 (±0.1) | 3.4 | 61.4 (±0.3) | 3.5 |
(14)
|
66.6 (±0.1) | 9.1 | 67.0 (±0.1) | 9.1 |
(15)
|
64.2 (±0.1) | 6.7 | 64.2 (±0.1) | 6.3 |
(16)
|
64.3 (±0.3) | 6.8 | 64.4 (±0.3) | 6.5 |
a
All values reported are derived from at least three melting temperature experiments with standard deviations indicated in parentheses (n.d. = not determined). ΔTm values are given as Tm(DNA/polyamide) −Tm(DNA).