Fig. 7.
Requirement for phosphorylation of Ser1700, Thr1704, and Ser1928 for PKA-mediated stimulation of CaV1.2 channel activity. (A) Coupling efficiency for the indicated constructs of CaV1.2Δ1800 + DCT in the presence or absence of 1 μM RO 31-8220, AKAP15, and 5 μM forskolin. Dashed black line indicates the mean current in unstimulated CaV1.2Δ1800 + DCT. *P < 0.05 or **P < 0.01 versus AKAP15 and forskolin (ITail, peak tail current; Q, total gating current; Vrev, reversal potential). (B and C) Current-voltage relationships of CaV1.2Δ1800 + DCT from (A) as indicated. Significance was determined by ANOVA.