Fig. 4.
Deactivation is faster for R120A, D163A, and R120A/D163A. A, macroscopic current responses to a 3-ms pulse of saturating GABA (indicated by arrow). Each mutant response is overlaid with the normalized wild-type response (light gray lines). B, summary of weighted time constants (τw) for deactivation, generated from biexponential fits of the macroscopic currents. τw is computed as Σai · τi/Σai, where ai and τi are the amplitude and time constant of component i, respectively. *, significant differences (P < 0.05) between control and mutant values were calculated using ANOVA with a Dunnett's post test.