TABLE 2.

Summary of macroscopic and microscopic parameters used for double-mutant cycle analysis

	Wild type	D163A	R120A	R120A/D163A	ΔΔGcoupling
					kcal/mol
EC50-GABA	65 μM	155 μM	920 μM	1600 μM	0.2
Deactivation τw	37.4 ms	15.6 ms*	3.5 ms*	3.5 ms*	0.5
kon	7.4 × 106 M−1 · s−1	4.3 × 106 M−1 · s−1*	8.3 × 105 M−1 · s−1*	5.0 × 105 M−1 · s−1*	0.03
koff1	8 s−1*	21 s−1*	118 s−1*	108 s−1*	0.63
koff2	271 s−1*	485 s−1*	1072 s−1*	1079 s−1*	0.34

^*P < 0.05, significant differences between control and mutant parameters were calculated using ANOVA with a Dunnett's post test.