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Phosphospecific antibody against MOPr (Ser363). Recombinant GST-MOPr-CT protein was incubated at 30°C for 30 min with PKC in the presence (lanes 1 and 3) or absence (lane 2) of ATP. Proteins (20 ng) were separated by 12% SDS-PAGE. Top, Western blot with the anti-pMOPrS363 in the absence (lanes 1 and 2) or the presence (lane 3) of phosphopeptide block. Bottom, the same membrane was stripped and reblotted with anti-MOPr antibody.
