Fig. 3.

Levels of APAP, APAP-GSH, APAP-S, and APAP-G in the plasma and LNG of WT and Cyp2a5-null mice at various times after APAP treatment. Two- to 3-month-old male mice, either B6 WT or Cyp2a5-null, were fasted overnight before a single injection of APAP (400 mg/kg i.p.). LNG and plasma were obtained from treated mice at 15 min, 1 h, or 2 h after APAP injection for determination of APAP (A and B; n = 6), APAP-GSH (C and D; n = 4), APAP-S (E and F; n = 4), and APAP-G (G and H; n = 4) levels. Values represent means ± S.D. **, P < 0.01; *, P < 0.05, Student's t test, for comparisons between WT and Cyp2a5-null mice.