TABLE 2.

In vitro metabolism of APAP by liver and LNG microsomes from WT and Cyp2a5-null mice

Rates of formation of the major in vitro APAP metabolite, APAP-GSH, were determined. Reaction mixtures contained 100 mM potassium phosphate buffer, pH 7.6, 0.5 mM APAP, 10 mM GSH, 0.5 mg/ml liver or LNG microsomal protein from 2-month-old male mice, and 1.0 mM NADPH. The values presented are means ± S.D. (n = 3).

Strain	Rates of APAP-GSH Formation
	Liver	LNG
	nmol · min−1 · mg protein−1
WT	0.46 ± 0.04	0.08 ± 0.01
Cyp2a5-null	0.43 ± 0.02	0.07 ± 0.01