Fig. 1.

Time course for effect of APAP on serum ALT, hepatic GSH, hepatic MnSOD activity, and hepatic MnSOD protein. Mice (n = 3) were treated with a 300 mg/kg dose of APAP and sacrificed at the indicated times, and serum and liver were collected. The 0 time is the saline-treated control mice. A, ALT levels in serum (hepatotoxicity). B, GSH levels in liver. C, MnSOD activity in liver. D, MnSOD protein levels in liver. Western blot analyses were performed on each homogenate using anti-MnSOD. GAPDH was used as a loading control. The data are presented as mean ± S.E. of the relative intensity. One representative gel is shown above the density values. *, significant difference from saline, p ≤ 0.05.