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. 2011 Mar 24;6(3):e17350. doi: 10.1371/journal.pone.0017350

Figure 4. Analysis of the promoter region and transcriptional start site for mphK.

Figure 4

(A) Intergenic region between mphR and mphK. The first nucleotide in the transcript is shown in bold. Arrows and dashed arrows indicate incomplete inverted repeats (named IR1, IR2, and IR3) and the translational start codons for mphR and mphK, respectively. Double-underlines mean the putative σ54-dependent −12/−24 promoter consensus sequence and the σ70-dependent −10/−35 promoter consensus sequences. An arrowhead with +1 indicates the transcriptional start site for mphK. A grey-shaded box indicates the putative ribosome-binding site for the mphK transcription. (B) Mapping of the transcriptional start site for mphK by primer extension. A sequence ladder was generated with the same primer (lanes T, A, C, and G).