Table 1. β-Galactosidase activities of mphK promoter-, mphR promoter-, or mphX promoter-lacZ transcriptional fusion in A. calcoaceticus PHEA-2 and mphR- and mphX-deletion mutants (A2R and A2X).
β-Galactosidase activity (Miller unit)a | ||
+Phenol | −Phenol | |
PHEA-2(PmphK-lacZ) | 336±33 | 34±2 |
A2R(PmphK-lacZ) | * | * |
A2X(PmphK-lacZ) | 807±58 | 30±2 |
PHEA-2(PmphR-lacZ) | 160±20 | 92±10 |
A2R(PmphR-lacZ) | 20±5 | 21±3 |
A2X(PmphR-lacZ) | 225±33 | 176±15 |
PHEA-2(PmphX-lacZ) | 332±50 | * |
A2R(PmphX-lacZ) | * | * |
A2X(PmphX-lacZ) | * | * |
Cells were grown in the presence (+) or absence (−) of 2.0 mM phenol and assayed for β-galactosidase activities as described in Materials and Methods. Values shown are means ± standard deviation of triplicate experiments.
*No detectable activity.