Table 3. Primers used in this study.
| Name | Sequence (5′-3′) a | Purpose |
| PR-F | AAGGATCCTTTAACCCAGCTTGATAACC | Amplification of mphR promoter region |
| PR-R | CGAATTCGGGATCCTCTGCATTTAAGTC | |
| PX-F | GTAAGCTTGGAGATAGTCGCACG | Amplification of mphX promoter region |
| PX-R | ACGGATCCACACCACATATGATTG | |
| R1-F | GCGAATTCAAGTCTTCAACATTAAC | Construction of mphR–deletion mutant |
| R1-R | AAGTCGACTTTGAGCAATTGTC | |
| R2-F | TGCGTATCGTCCCGTTGTA | |
| R2-R | TCGGCTGTGTCCTGTAAAC | |
| N1-F | ATGAATTCGCCACGTTGAACAAAC | Construction of mphN–deletion mutant |
| N1-R | TTGTCGACGATTGATCATCCTTAAATC | |
| N2-F | ATGTCGACAAGCATGGCTACCTG | |
| N2-R | TTGGATCCAGCTTGGAATTCGATTG | |
| X1-F | GCAGCGACTACTTATGTGC | Construction of mphX–deletion mutant |
| X1-R | ATTTGGAAGGCAGAACTCCT | |
| X2-F | ATGTCGACTATAGCTTGGGTTATC | |
| X2-R | TTGGATCCGTGAATTGATGAAGATG | |
| MphR188-F | TCGAGCCATGAGTGATCTGTT | Amplification of the 380-bp mphR-mphK intergenic region |
| MphK207-R | GTACTCCATCAAGACATGGTC | |
| M13-fwd | CACGACGTTGTAAAACGAC | Amplification of the 380-bp mphR-mphK intergenic region |
| M13-rev | GGATAACAATTTCACACAGG |
a
Restriction sites for BamHI (5′-GGATCC-3′), HindIII (5′-AAGCTT-3′), EcoRI (5′-GAATTC-3′), and SalI (5′-GTCGAC-3′) are shown in bold and italic.