Skip to main content
. 2011 Mar 24;6(3):e17990. doi: 10.1371/journal.pone.0017990

Figure 2. PCR amplification of ITS and TRI genes.

Figure 2

Panel A: Agarose gel electrophoresis showing PCR products of ITS and TRI from four F. sambucinum strains T3, T5, T6 and T8 isolated from naturally infected potato. Lanes (2–5) show ITS PCR products from strains T3, T5, T6 and T8 respectively. Lanes (7–10) and (12–15) show TRI5 and TRI101 PCR products from T3, T5, T6 and T8 respectively. Lanes, 1, 6 and 11 are negative controls. Lane M, shows 1 Kb ladder. The PCR products for TRI4 (1.4 kb; lanes 7–10) and for TRI11 (1.5 kb; lanes 12–15) are of the expected size. Panel B: shows PCR patterns of TRI3 (lanes 2–5), TRI4 (lanes 7–10 and 12–15) from strains T3, T5, T6 and T8, respectively. Lanes, 1, 6 and 11 are negative controls. Lane M shows 1 kb ladder.