Table 1. Primers sets used for PCR and RT-PCR experiments.
Primer | Nucleotide sequences (5′-3′) | Target gene | Reaction |
ITS1 | TCCGTAGGTGAACCTGCGG | ITS | PCR |
ITS4 | TCCTCCGCTTATTGATATGC | ITS | PCR |
1912 | TGTGTMGGYGCWGAGGCVATYGTTGG | TRI3 | PCR |
1914 | ACRGCAGCRGTCTGRCACATGGCGTA | TRI3 | PCR |
1450 | ACCTTGAGTTCTACCATGAAGTCATC | TRI4 | PCR |
1455 | GCACTGTCTAGARCCCTGAGAGAAGT | TRI4 | PCR |
1558 | GGCATGGTCGTGTACTCTTGGGTCAAGGT | TRI5 | PCR |
1559 | GCCTGMYCAWAGAAYTTGCRGAACTT | TRI5 | PCR |
1482 | CACACYCTCCTSATGCTYTGTGGACT | TRI11 | PCR |
1483 | TCCCAMACTGTYCTYGCCAGCATCAT | TRI11 | PCR |
109 | CCATGGGTCGCRGGCCARGTSAA | TRI101 | PCR |
178 | AACTCSCCRTCIGGYTTYTTNGGCAT | TRI101 | PCR |
ß-tubulin-F | GCCATGAAAGGAGGTTGAGGA | Ref. gene | RT-PCR |
ß-tubulin-R | AAGCCTTGCGTCGGAACATA | Ref. gene | RT-PCR |
EF 1α-F | GTACGCCTGGGTTCTTGACA | Ref. gene | RT-PCR |
EF 1α-R | GAGCGTCTGGTAGGCATGTTAG | Ref. gene | RT-PCR |
TRI4-F | GCCACTGCTGCTACTGTTG | TRI4 | RT-PCR |
TRI4-R | GGTCGTTGTCCAGATGTTCTTG | TRI4 | RT-PCR |
TRI5-F | TGGAGAACTGGATGGTCTGG | TRI5 | RT-PCR |
TRI5-R | GACATAGCCGTGCATGAAGC | TRI5 | RT-PCR |
TRI6-F | AGTGCCAAGTCAGCTCATCG | TRI6 | RT-PCR |
TRI6-R | GAGCACGATCCTTGCGAGTT | TRI6 | RT-PCR |
TRI13-F | CTGCGGTGGAACCGCTGGTA | TRI13 | RT-PCR |
TRI13-R | ACACTGGCGTTGTCCGTAAG | TRI13 | RT-PCR |
TRI101-F | ATCGCCAACGAACCACTTG | TRI101 | RT-PCR |
TRI101-R | TGATGCTGCTTGACGGATTC | TRI101 | RT-PCR |