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. 2011 Feb 17;12:9. doi: 10.1186/1471-2091-12-9

Figure 2.

Figure 2

Spectral analysis of purified B. breve MCRA protein. SDS PAGE analysis of B. breve MCRA protein purified on Ni-NTA column. Lane 1, molecular weight marker, lane 2, B. breve MCRA protein of apparent MW of 66 kDa, lane 3, immunoblot with anti-His tag antibody shows the absence of significant proteolytic degradation in purified B. breve MCRA. B UV-Vis spectrum of B. breve MCRA protein (black) and the supernatant after heat precipitation (red), showing flavin-like absorbance. For the reference spectra of 10 μM FAD (blue) and FMN (magenta) are shown.