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Primers used in this work. Restriction sites are underlined
| Primer | Restriction site | Resulting construct | Sequence |
|---|---|---|---|
| EMY01 | PstI | pEMYL1 | 5 '- AACTGCAGTGGATTGTCAATTCATCCCC- 3 ' |
| EMY02 | HindIII | 5' - CCCCAAGCTTGCCCGATTATGCGAACGGCT- 3' | |
| EMY03 | NdeI | pEMYC2 | 5 '- AACATATGTGGATTGTCAATTCATCCCC- 3 ' |
| EMY04 | AvrII | 5' - CCCCCCTAGGGCCCGATTATGCGAACGGCT- 3' | |
| BBI_fw2 | NdeI | pBBI2 | 5 ' - AACATATGTACTACAGCAGCGGCAACTATGAG- 3 ' |
| BBI_rv2 | XhoI | 5 ' - TCTCGAGTTAGATCACATGGTATTCGCGTAGCAGGGTAGC- 3 ' | |
