Table 2.

Levels of Hepatic rGSH, Its Disulfide (GSSG), Cysteine, and SAMe at Normal State (0h), 4 and 18 Hours After LPS Administration, Measured by HPLC as nmol/g of Tissue

Parameters	CHOW	CYSPUFA	CASN
Normal hepatic rGSH, nmol/g	5087 ± 269*	5301 ± 416	5778 ± 132*
rGSH loss nmol/g, 4 hours	−952 ± 47**	−374 ± 85**	−650 ± 180*
Hepatic GSSG, nmol/g	102 ± 7.8*	82 ± 6.6*	87.5 ± 10
Cys nmol/g, 4 hours	199 ± 41*	246 ± 36*	250 ± 27
Cys nmol/g, 18 hours	181 ± 13*	255 ± 21*	253 ± 16
Hepatic SAMe, nmol/g, 18 hours	56.9 ± 6**	65.2 ± 2	79.1 ± 3**
HCT, 4 hours	42.0 ± 2.5	43.7 ± 2.1*	39.3 ± 1.2*
Serum albumin density, 0 hours	1034 ± 1	1034 ± 1	1035 ± 1
Serum albumin density, 4 hours	1030 ± 1	1032 ± 2	1032 ± 1
IL-6, pg/mL, 4 hours	581 ± 5	579 ± 4	546 ± 24
IL-6, pg/mL, 18 hours	29 ± 2.6	35 ± 3.9	32 ± 3.8
IFNγ, pg/mL, 4 hours	2993 ± 51*	2602 ± 247*	2950 ± 54
IFNγ, pg/mL, 18 hours	209 ± 67	237 ± 81	275 ± 84
TNFα, pg/mL, 4 hours	258 ± 43	317 ± 85	275 ± 105
TNFα, pg/mL, 18 hoursa	7.4 ± 1.6	9.07 ± 2.2	5.3 ± 1.8

HCT and blood cytokines measured with the enzyme-linked immunosorbent assay (ELISA) technique. Data are expressed as mean ± SEM (ordinary and repeated-measures ANOVA followed by post hoc analysis). Cys, cysteine; GSSG, oxidized glutathione; HCT, hematocrit; HPLC, high-performance liquid chromatography; IL-6, interleukin-6; IFNγ, interferon γ; rGSH, reduced glutathione; SAMe, S-adenosylmethionine; TNFα, tumor necrosis factor α.

^aReturned to normal level.

^*P < .05.

^**P < .01.