Figure 1.

The protein content of glial fibrillary acidic protein (GFAP) increases as cultured primary astroglia mature, whereas vimentin content remains constant. (A) Western blot analysis of true control primary glia cultures shows that the GFAP protein content in day-in-vitro (DIV) 7 and 15 cultures are increased 2.8- and 4-fold, respectively, as compared to DIV4 cultures. Vimentin protein levels remained unchanged in DIV7 and 15 cultures. The values shown are means ± SEM of the densitometric analysis from 4 different primary cultures per time point. (B) Ratios of GFAP to vimentin densitometric values at the 3 time points. Corresponding representative Western blots are shown underneath the graphs.