Figure 4.
Isoflurane has no effect on glial fibrillary acidic protein (GFAP), vimentin, or total actin protein content in immature primary astroglia. Isoflurane treatment at day-in-vitro (DIV) 4 (3% for 24 hours) has no effect on the protein levels of GFAP, vimentin, or total actin when measured at DIV7 (2 days post-treatment). Experimental cultures were compared with age-matched sham control cultures (designated as 100%) (n = 4 per data point). Corresponding representative Western blots are shown below the graph.