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Isoflurane induces a decrease in levels of endogenous RhoA in immature primary astroglia. Isoflurane treatment at day-in-vitro (DIV) 4 (3% for 24 hours) causes a decrease of approximately 75% in the protein content of endogenous RhoA when measured at DIV7 (2 days post-treatment) (*p < 0.01; n = 4 per data point). Experimental cultures were compared with age-matched sham control cultures (noted as 100%). Corresponding representative Western blots are shown below the graph.
