Figure 4. Higher tumor burden with loss of E-Cadherin and gain of mesenchymal and metastatic proteins in BxPC3 MUC1 versus Y0 and Neo tumors.
A. In vivo tumor growth in nude mice. Significantly higher tumor burden in BxPC3. MUC1 versus Neo and Y0 tumors (*p < 0.001). B. MUC1 expression by Western blotting using the MUC1 TR and CT antibodies C. Tumor cells cultured from whole blood of tumor-bearing mice. Circulating tumor cells detected in the blood of mice bearing the BxPC3 MUC1 but not BxPC3 Y0 or Neo tumors. D. Expression of E-Cadherin in primary tumors by Western blotting. E. Expression of Slug, Snail, and Vimentin in primary tumors by Western blotting. F. High levels of pro-metastatic and pro-angiogenic proteins detected in the BXPC3 MUC1 tumor lysate. Fold change in levels of various factors in BxPC3 MUC1 tumor lysate compared to Y0 and Neo, G. Expression of VEGF and E-cadherin by IHC. Images were taken at 400X magnification. Similar results with n=3 mice were obtained.