Fig. 5.

Effects of 1,8-cineole on the proliferation and elongation of BY-2 cells. A The effects of a range of doses of 1,8-cineole on the proliferation of BY-2 cells cultured in D-medium are shown. The mitotic index was examined on d 2. The average values from three independent experiments are shown. Vertical bars represent standard errors. B The effects of a range of doses of 1,8-cineole on elongation (top) and starch accumulation (bottom) in BY-2 cells cultured in B-medium. Cell length and starch content were examined on d 2. The average values from three independent experiments are shown. Vertical bars represent standard errors. In A and B, 1,8-cineole was applied through the direct-addition method only. The estimated final concentrations of 1,8-cineole in the airspace (gas phase) were 0 to 240 μM, while the initial concentrations in the culture medium (liquid phase) immediately after application were 25 times higher. IC₅₀ values estimated from the data are shown in respective graphs