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. Author manuscript; available in PMC: 2011 Aug 1.
Published in final edited form as: Cancer Res. 2010 Jul 14;70(15):6283–6292. doi: 10.1158/0008-5472.CAN-10-0412

Figure 2. Transplantation of vascular proangiogenic cells (VPCs) promote maturation of the tumor vasculature resulted unreduced hypoxic area in spontaneously developed PDAC.

Figure 2

Sequential VPC transplantation (5 ×105; twice) was performed in Pdx1-Cre;LSL-KrasG12D;p53lox/+ mice (12 weeks old), and mice were sacrificed 2 weeks after transplantation. (A) Double immunofluorescent staining with NG2 with CD31 in Zn-fixed sections. Arrowheads indicate NG2+ pericyte-covered microvessels. (B) Double immunofluorescent staining with anti-Hypoxyprobe-1 with anti-CD31 in frozen sections. Scale bars; 100 μm.