Fig. 1.

Expression of UBpcAMA-1 in mammalian COS7 cells. (A) A map showing the construction of the plasmid using the mammalian expression vector pcDNA 3.1(-), including the AMA-1 insert. The plasmid miniprep products from DNA-ubiquitin fused vector pcDNA 3.1(-) were cut by enzyme digestion with Xho I and Apa I. The antigen was cloned into the pcDNA 3.1(-) vector, and the expression plasmid was constructed. The vector was inserted with the mutant ubiquitin gene, so that the expression plasmid was expected to be generated by the ubiquitin-proteasome pathway in mammalian cells. (B) COS-7 cells transfected with UBpcAMA-1 using lipofectamine after immunofluorescence staining. The AMA-1 antigen was successfully expressed in cultured COS-7 cells. (C) Western blot analysis of the protein expression of the plasmid DNA in transfected COS7 cells. The PvAMA-1 recombinant protein was approximately 56.8 kDa.