Fig. 4.
Requirement of the α1C-CaL COOH terminus for association of α1C-CaL with c-Src or β1-integrin. A: representative blot comparing WT- and truncated α1C-CaL immunoprecipitated with either anti-c-Src, anti-β1-integrin Ab, or mouse IgG, then probed for α1C. α1C was only detected in lysates immunoprecipitated with anti-c-Src (lanes 2 and 7) or anti-β1-integrin Ab (lanes 1 and 8) but not with mouse IgG (lanes 3 and 6). Relatively lower amounts of α1C-CaL were pulled down by anti-c-Src or anti-β1-integrin Ab in truncated α1C-CaL (lanes 7 and 8, respectively), compared with WT. B: the same blot in A after stripping and reprobing for β1-integrin as a control for IP and immunoblotting. C: summary graph of the relative levels of immunoprecipitated α1C in cells expressing WT- or truncated α1C-CaL. Values were obtained as described in methods and are based on the average of at least three experiments. *P < 0.05 vs. WT + anti-β1-integrin Ab or WT + anti-c-Src Ab.