Fig. 5.
S1901 and Y2122 in α1C-CaL are required for α1C-CaL association with β1-integrin or c-Src. A: representative blot comparing WT α1C-CaL and S1901A/Y2122F α1C-CaL immunoprecipitated with either anti-c-Src, anti-phosphorylated tyrosine (Tyr-Pi), or anti-β1-integrin Ab, and probed for α1C. Relatively lower amounts of α1C-CaL appear to be pulled down by anti-c-Src, anti-Tyr-Pi, or anti-β1-integrin Ab (lanes 1, 2, and 3, respectively) in S1901A/Y2122F α1C-CaL-expressing cells, compared with WT-α1C-CaL. B: the same blot in A after stripping and reprobing for β1-integrin. C: summary graph showing the relative levels of immunoprecipitated α1C in WT- or S1901A/Y2122F-CaL. Values were obtained as described in methods and are based on the average of at least three experiments. *P < 0.05 vs. WT + anti-β1-integrin Ab, WT + anti-Tyr-Pi Ab, or WT + anti-c-Src Ab.