Table 1.
Semiquantitative analysis of CaL association with β1-integrin
| Rr, % of Pax + Vin | R, % of Pax + Vin | ICA, % | ICA, % of Pax + Vin | |
|---|---|---|---|---|
| Paxillin-vinculin | 100 | 100 | 0.251 ± 0.021 | 100 |
| WT | 60.6 ± 2.7 | 88.5 ± 1.5 | 0.22 ± 0.009 | 85.1 ± 4.2 |
| Truncated-α1C | 59.2 ± 4.8 | 87.9 ± 2.6 | 0.211 ± 0.01 | 84.1 ± 5.0 |
| S1901A/Y2122F | 56.5 ± 4.5 | 84.5 ± 2.5 | 0.21 ± 0.011 | 84.8 ± 5.3 |
| ΔP1/ΔP2 | 55.4 ± 5.1 | 84.1 ± 1.7 | 0.185 ± 0.013 | 75.2 ± 7.4 |
Values are means ± SE, taken from at least eight cells in either wild-type (WT) or mutant L-type voltage-gated Cav1.2 calcium channel (CaL) per experiment. The degree of CaL association with β1-integrin was normalized using the association of paxillin (Pax) and vinculin (Vin) as a reference and set as 100% for Pearson's, Mander's, or intensity correlation analysis (ICA). The value of CaL association with β1-integrin in WT, truncated α1C-, S1901A/Y2122F, or ΔP1/ΔP2-CaL is presented as the percentage ± SE of paxillin-vinculin association. Rr, Pearson's coefficient; R, Mander's coefficient. Statistical analysis was performed on the basis of the average from at least four individual experiments.