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. 2010 Dec 1;300(3):C406–C415. doi: 10.1152/ajpcell.00403.2010

Fig. 6.

Fig. 6.

Human keratinocyte HaCaT cells activate matriptase in response to extracellular acidity. Immortalized human keratinocyte HaCaT cells were exposed to a pH 6.0 buffer or basal medium as negative control for 30 min. Cell lysates were prepared and analyzed by immunoblot analysis for total matriptase (total MTP), HAI-1, and activated matriptase (activated MTP). Matriptase and prostasin were robustly activated and rapidly inhibited by HAI-1 to form a 120-kDa complex and an 85-kDa complex, respectively. Both complexes were clearly detected by HAI-1 MAb.