Fig. 6.

Single-channel recordings confirm that both β-subunits prolong bursting, even in the absence of Ca²⁺. Single-channel tracings from excised inside-out patches expressing Slo, Slo and β₄, and Slo and β₁ in 0 μM (A) and 1 μM (B) Ca²⁺ are shown. As is evident, both β-subunits increase bursting of the channel even in nominally Ca²⁺-free solutions, with β₁ having a more marked effect. Open (o) and closed (c) states are indicated.