Fig. 3.

Effect of dopamine on Na⁺-K⁺-ATPase α1-subunit association with dopamine 1 receptor, NHERF-1, and PKC-ζ. Vector transfected WT and NHERF-1-deficient OK (OKH) cells, or OKH cells transfected with full-length NHERF-1 (NF), PDZ-1 (Z1)-mutated, or PDZ-2 (Z2)-mutated NHERF-1 were treated for 15 min with 1 μM dopamine at 37°C. Cells were lysed and dopamine 1 receptor (D1 receptor, A), NHERF-1 (B), or Na⁺-K⁺-ATPase α1-subunit (C) was immunoprecipitated and analyzed by Western blot analysis using antibodies against D1 receptor, NHERF-1, Na⁺-K⁺-ATPase α1-subunit, or PKC-ζ. A representative blot from three independent experiments is shown. Bar diagram represent data as A.U. means ± SE from three independent experiments. *P < 0.05 by ANOVA followed by Bonferroni's analysis from the respective vehicle-treated cells.