Fig. 6.

Immunofluorescence of collagen-1 (Coll-1), discoidin domain receptor 2 (DDR2), and heat shock protein 47 (HSP47) in EC cultured on microfibrils isolated from C57BL/6J, PBS-treated Tsk^−/+ mice, and D-4F-treated Tsk^−/+ mice. A–C: increased expression (bright green) of collagen-I (A), DDR2 (B), and HSP47 (C) in EC cultured on microfibrils isolated from Tsk^−/+ mice compared with expression levels in EC cultured on microfibrils isolated from C57BL/6J and D-4F-treated Tsk^−/+ mice. Fluorescence intensity is depicted below each panel. Fluorescence intensity was measured by determining pixel intensity of each cell (Tsk^−/+ is significantly different from the other groups, #P < 0.05).