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. 2010 Dec 9;300(3):C496–C505. doi: 10.1152/ajpcell.00292.2010

Fig. 2.

Fig. 2.

Effect of NHE3 silencing on SGLT1 message and protein expression in IEC-18 cells. Quantitative RT-PCR (RTQ-PCR) analysis of NHE3 and SGLT1 in NHE3 siRNA-transfected IEC-18 cells is shown. A: as expected, in NHE3-silenced cells, NHE3 message was significantly reduced compared with control IEC-18 cells transfected with negative control siRNA. B: however, in NHE3-silenced cells, SGLT1 message was increased significantly compared with control IEC-18 cells. Thus, silencing NHE3 stimulates SGLT1 mRNA levels. C: in NHE3-silenced cells, NHE3 protein was significantly reduced compared with IEC-18 cells transfected with negative control siRNA. D: however, in NHE3-silenced cells, SGLT1 protein was increased significantly compared with IEC-18 cells transfected with negative control siRNA. Ezrin control is for both blots. Thus, silencing NHE3 stimulates SGLT1 at the protein level.