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. 2010 Dec 9;300(3):C496–C505. doi: 10.1152/ajpcell.00292.2010

Fig. 5.

Fig. 5.

Effect of SGLT1 silencing on NHE3 message and protein expression in IEC-18 cells. RTQ-PCR analysis of NHE3 and SGLT1 in SGLT1 siRNA-transfected IEC-18 cells is shown. A: as expected, in SGLT1-silenced cells, SGLT1 message was significantly reduced compared with control IEC-18 cells transfected with negative control siRNA. B: however, in SGLT1-silenced cells, NHE3 message was increased significantly compared with control IEC-18 cells. Thus, silencing SGLT1 stimulates NHE3 mRNA levels. C: in SGLT1-silenced cells, SGLT1 protein was significantly reduced compared with IEC-18 cells transfected with negative control siRNA. D: however, in SGLT1-silenced cells, NHE3 protein was increased significantly compared with IEC-18 cells transfected with negative control siRNA. A representive blot for each condition and densitometric analysis of four blots are given. Ezrin control is for both blots. Thus, silencing NHE3 stimulates SGLT1 at the protein level.