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. 2010 Dec 9;300(3):C496–C505. doi: 10.1152/ajpcell.00292.2010

Fig. 6.

Fig. 6.

Immunocytochemical analysis of the effect of SGLT1 silencing on NHE3 protein in IEC-18 cells. IEC-18 cells transfected with negative control or SGLT1 siRNA were subjected to immunocytochemical analysis using NHE3 and SGLT1-specific primary antibodies. A and C: top views of IEC-18 cells treated with negative control siRNA expressing SGLT1 (A) and NHE3 (C). B and D: top views of IEC-18 cells expressing SGLT1 (B) and NHE3 (D) after transfection with SGLT1 siRNA. E and F: quantitative data of the fluorescence intensities of negative control and SGLT1 siRNA-transfected cells (n = 4, *P < 0.05). These data demonstrated that siRNA-mediated reduction in NHE3 in IEC-18 cells enhances SGLT1 expression.