Fig. 5.
Effect of Cav3.1 downregulation on cyclin D1 transcription. PASMCs were infected for 5 days with AdX-scr or AdX-Cav3.1sh and maintained in 0.1% FBS to block further proliferation and then stimulated with 100 ng/ml IGF-I. Cells were harvested at 2 h (n = 4), 4 h (n = 5), and 6 h (n = 4), and RQ of cyclin D1 was calculated vs. control (T = 0, AdX-scr infected) PASMC. Relative levels of Cav3.1 and IGF receptor (IGF-R1) mRNAs were assayed in the same samples. Values are means ± SE. Statistical significance is calculated on dCt values using paired t-test. *P < 0.05 vs. T = 0, AdX-scr control PASMCs. #P < 0.05, ##P < .01 vs. time-matched Adx-scr.