Fig. 4.
Acute ex vivo effects of P4 and/or E2 on mEH2O2 in permeabilized human female myofibers. Rates of mEH2O2 measured in saponin-permeabilized myofibers from lean, healthy women in menstrual cycle follicular phase (days 1–10). Permeabilized fibers were incubated in either DMSO (<1.5%, vehicle), 1.4 nM E2, 60 nM P4, or both (E2 + P4). Substrate conditions were, in the presence of 10 μM oligomycin: 25 μM palmitoylcarnitine + 1 mM malate (P-C/M); P-C/M + 2 mM glutamate (+G); P-C/MG + 3 mM succinate (+S); and P-C/MGS + 10 mM glycerophosphate (+Gp). Results are means ± SE (n = 5). *Less than control (DMSO), P < 0.05; **P < 0.01; ***P < 0.001; †less than E2, P < 0.05.