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. Author manuscript; available in PMC: 2011 Oct 1.
Published in final edited form as: J Immunol. 2011 Feb 28;186(7):3874–3881. doi: 10.4049/jimmunol.1003231

Figure 4. Differential contribution of B7-mediated costimulation on the phenotype of stable and inflationary MCMV-specific CD8 T cells.

Figure 4

WT and B7.1/2−/− mice were infected with MCMV. (A) Flow plots show the cell surface phenotype of M45- and M38-tetramer positive (Tet+) CD8 T cells at day 8 post-infection. CD8 T cells were analyzed for tetramer reactivity, CD44, CD62L and CD127 expression. (B) The cell surface phenotype of M45-, M38- and IE3-Tet+ positive CD8 T cells was analyzed at day 40 post-infection. Plots were gated on tetramer positive CD8 T cells and analyzed for CD27 and CD62L expression. (C) At day 100 post-infection, MCMV-specific CD8 T cells were analyzed for tetramer binding or intracellular expression of IFN-γ after restimulation with the indicated class I epitopes. Graph shows the percentage of Tet+ versus IFN-γ+ CD8 T cell population at day 100 post-infection. (D) At day 100 post-infection, M45- and IE3-Tet+ CD8 T cells in the spleen were analyzed for expression of PD-1 and CD27. Data shown are representative of two independent experiments.