Figure 7. B7 costimulation is not required for cytolytic function of MCMV-specific CD8 T cells.

Peptide-pulsed target cells were differentially labeled with two separate fluorescent dyes in order to track 4 separate populations (no peptide, M45-, M38- and IE3-peptide pulsed), and were transferred into uninfected mice (naïve control) or into WT and B7.1/2^−/− mice infected with MCMV for 8 or 40 days. The top panels represent a typical analysis of target cells recovered from the spleen after transfer (shown is day 8). Numbers indicate the percent killing of labeled targets after normalization (see methods). Graphs summarize the killing efficiency and show the averaged values. 4-7 mice were used per group and each experiment was repeated twice. Statistical significance was determined by Student’s t test (* indicates p<0.05 and ** p<0.005). Data shown are representative of two independent experiments.