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. 1987 Nov 11;15(21):8773–8781. doi: 10.1093/nar/15.21.8773

Specific labelling of the active site of T7 RNA polymerase.

A R Schäffner 1, E D Jorgensen 1, W T McAllister 1, G R Hartmann 1
PMCID: PMC306404  PMID: 3684573

Abstract

We describe a method for specifically labelling T7 RNA polymerase at (or near) the active site. Enzyme molecules that have been modified by covalent attachment of a benzaldehyde nucleotide derivative in the presence of template DNA are subsequently incubated with radioactively labelled nucleoside triphosphates. Labelling of the enzyme occurs as a result of the formation of the first phosphodiester bond. The labelling is template-directed and the expected specificity of initiation at individual T7 promoters is observed. The label has been localized to an 80 kd tryptic fragment that contains the carboxy-terminal portion of the enzyme.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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