FIG. 1.

ER stress induces LIPIN2 expression in vivo. A: Wild-type C57BL/6 mice were injected intraperitoneally with either DMSO or tunicamycin (2.5 μg per g body wt, n = 4 per group), and livers were collected 2 h post-injection. Quantitative PCR analysis was performed with hepatic RNAs. B: Saline (as a vehicle) or LPS (3 μg per g body wt, n = 5 per each group) was injected intraperitoneally, and livers were collected 8 h post-injection. Quantitative PCR analysis was performed with hepatic RNAs. C: Western blot analysis was performed with lysates from mouse livers as in A. Representative data from three independent experiments are shown. D: Western blot analysis was performed with lysates from mouse livers as in B. Representative data from three independent experiments are shown. E and F: Hepatic RNAs for quantitative PCR analysis (E) and lysates for Western blot assay (F) were prepared from mice on either normal chow diet or high-fat diet (60 kcal% fat for 12 weeks, D12492 [Research Diets] n = 5 per group). Representative data from three independent experiments are shown. Error bars indicate SEM. Statistical significance was assessed by two-tailed Student t test. *P < 0.05; **P < 0.01. sXBP1, spliced XBP1; uXBP1, unspliced XBP1.