FIG. 3.

Effect of TNF-α on human skeletal muscle cell cytokine secretion. A: Human myotubes were cultured 24 h ± 20 ng/mL TNF-α, and cytokine levels in the conditioned media were monitored by hybridization to human chemokine + cytokine array membranes. White circles show modified protein levels between the two conditions on representative membranes from three different experiments. B: Each membrane was quantified using Multi Gauge software, and the intensity was normalized to internal positive controls for comparison. n = 3 independent experiments. TM, medium from human skeletal muscle cells cultured 24 h with TNF-α; CM, medium from human skeletal muscle cells cultured 24 h without TNF-α.