TABLE 1.
Primary antibodies used for different experiments in this study
| Antibody | Catalog no. | Vendor | Host | Working dilution |
|
|---|---|---|---|---|---|
| IF | WB | ||||
| Anti-Iba1 |
019–19741 |
Wako |
Rabbit |
1:200 |
— |
| Anti-OX-42 |
201801 |
BioLegend (San Diego, CA) |
Mouse |
1:200 |
— |
| Antiphospho-ERK |
9106 |
Cell Signaling Technology Inc. (Danvers, MA) |
Mouse |
1:400 |
1:2,000 |
| Antiphospho-P38 |
4631 |
Cell Signaling Technology Inc. |
Rabbit |
1:100 |
1:1,000 |
| Anti-GFAP |
AB5541 |
Millipore (Billerica, MA) |
Chicken |
1:500 |
— |
| Anti-AGA |
A717 |
Exocell Inc. (Philadelphia, PA) |
Mouse |
1:50 |
1:500 |
| Anti-albumin |
PA1–10298 |
Thermo Fisher Scientific (Waltham, MA) |
Rabbit |
— |
1:2,000 |
| Antiactin |
A2066 |
Sigma-Aldrich Co. (St. Louis, MO) |
Rabbit |
— |
1:2,000 |
| Anti-ERK |
9102 |
Cell Signaling Technology |
Rabbit |
— |
1:1,000 |
| Anti-P38 |
9212 |
Cell Signaling Technology |
Rabbit |
— |
1:1,000 |
| Antiphospho-JNK |
9255 |
Cell Signaling Technology |
Mouse |
— |
1:2,000 |
| Anti-JNK |
9252 |
Cell Signaling Technology |
Rabbit |
— |
1:1,000 |
| Anti-rabbit (Texas red or Oregon green) |
A21072 |
Invitrogen (Carlsbad, CA) |
Goat |
1:500 |
— |
| 737677 |
Chicken |
||||
| Anti-mouse (Texas red or Oregon green) |
737674 |
Invitrogen |
Chicken |
1:500 |
— |
| A11017 |
Goat |
||||
| Antichicken (Cya5) |
92590 |
Millipore |
Donkey |
1:500 |
— |
| Anti-rabbit HRP |
7074 |
Cell Signaling Technology |
Goat |
— |
1:10,000 |
| Anti-mouse HRP | 7076 | Cell Signaling Technology | Goat | — | 1:10,000 |
HRP, horseradish peroxidase; IF, immunofluorescence; WB, Western blot.